Newsletters Archives - Page 2 of 2

First Publication from “Oxygen Measurement Core”

Newsletters / May 9, 2021

Did you know that JIVA-25™ can provide in vivo partial oxygen pressure (pO2) maps of an implanted devices in a rodent? The pO2 maps provide a crucial missing information to assess the success of all transplanted devices. Reach out to us if you are interested in performing such study in collaboration with us at our "Oxygen Measurement Core" facility or at your lab by bringing a JIVA-25™ to your lab. Looking forward to talk to you about what excites you in your journey of developing new cell- and tissue-based therapies!

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In Vivo Oxygen Imaging of Islet Encapsulation Devices using JIVA-25™

Newsletters / May 9, 2021

In Vivo Oxygen Imaging of Islet Encapsulation Devices using JIVA-25™ Read More »

O2M at the “Society for Biomaterials 2021 Annual Meeting”

Newsletters / April 7, 2021

Rapid Fire Presentations

558: In Vitro pO2 Measurement of Islet Encapsulation Devices in Oxygen Measurement Core
(In collaboration with the University of Chicago, University of Arizona, Cornell University, University of Florida, and McGill University)

This work is the outcome of JDRF-supported “Oxygen Measurement Core” facility established at O2M Technologies. We performed in vitro and in vivo pO₂ measurements of acellular and cell loaded islet cell transplantation devices originated from the core members' labs. These devices varied in shape, size, biomaterials, and oxygen profile. We will present key data from these measurements.

559: Trityl Radical OX071, an EPR Oxygen Imaging Spin Probe, Is Non-Toxic to Cells
(In collaboration with the University of Chicago)

Our experimental results using many different cell types showed that trityl OX071, the oxygen reporting molecule for electron paramagnetic resonance oxygen imaging is non-toxic to cells, suggesting it can be used as an in vitro or in vivo spin probe for pO₂measurements in cell-based therapies.

560: Methodology for Biomaterial Oxygen Imaging Using Trityl Based Pulse Electron Paramagnetic Resonance
(In collaboration with the University of Chicago)

Oxygen is an important indicator of the physiologic state of tissues and bioartificial devices. Although oxygen point measurements using polarographic needle electrodes or luminescence-based optical sensors can reveal local oxygenation, three-dimensional oxygen maps deliver the complete information and thus can better assist the development of artificial cell and tissue replacement devices. This is especially important for samples where metabolically active cells can create steep oxygen gradients on the scale of hundreds of microns. For optically opaque samples magnetic resonance methods are found to be the most suitable. Pulse electron paramagnetic resonance oxygen imaging (EPROI) is an emerging technique to provide three-dimensional partial oxygen pressure (pO₂) maps of tissues and biological samples.

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340: Synergistic Effect of Placental Membrane Extract and Hypoxia on Human Adipocyte Differentiation

(In collaboration with University of Illinois College of Medicine Rockford)

One of the major drawbacks associated with autologous fat grafting is unpredictable graft retention. With a potential volume loss of 30-70% at one year, identifying methods to improve graft survival are relevant. Each step along the way, i.e., harvesting, processing, and transplantation have opportunities to impact the success of this procedure. Our synthesized Gtn-FA hydrogel crosslinked with laccase effectively produces a hypoxic environment as validated by EPROI. Gene expression data showed that key gene markers for differentiation and angiogenesis were significantly upregulated compared to baseline, further validating the efficacy of ACM supplementation as a novel way to promote adipocyte survival and retention.

O2M at the “Society for Biomaterials 2021 Annual Meeting” Read More »

Application of Oxygen Imaging to Oxygen Microbubble Therapy

Newsletters / December 4, 2020

Hypoxia severely limits the efficacy of radiotherapy, chemotherapy, and immunotherapy. This results in diminished success in treating cancer. Despite consistent research in this field and treatments with promising anti-hypoxia mechanisms, there is no treatment for ameliorating hypoxia.

Electron paramagnetic resonance oxygen imaging (EPROI) provides 3D oxygen maps of tissues at high resolution. At Duke University, JIVA-25 is currently being utilized in radiation biology applications, investigating a promising radiation sensitizer in the E0771 murine flank tumor model. Oxygen microbubbles are venously-introduced and burst in the tumor via ultrasound, providing a bolus of oxygen. In vivo pilot studies have shown an increase in hemoglobin saturation in murine tumor models; however, to fully elucidate the role of oxygen microbubbles in alleviating hypoxia and to determine the ideal timing of prospective radiotherapy, the increase in tissue pO2 must be directly quantified spatially and temporally. These described experiments, while focusing on the radiation biology field, will provide a preclinical imaging paradigm for quantifying hypoxia in vivo that is applicable to any research that requires precise evidence of tissue pO2.

JIVA-25, The Next Big Thing in Oxygen Imaging

O2M Technologies proudly presents JIVA-25, the world's first dedicated oxygen imager for preclinical research. JIVA-25 operates at 25 mT magnetic field and uses pulse electron paramagnetic resonance imaging methodologies such as inversion recovery electron spin echo (IRESE) and single point imaging (SPI) for superior oxygen images. JIVA-25 is a portable instrument with a small footprint and modest power requirements that can be installed in any laboratory or animal room.

JIVA-25 measures the relaxation maps of a water-soluble oxygen-reporting trityl molecule that distributes in tissues upon injection and converts them into oxygen images. Trityls (OX063 or its deuterated version OX071, also known as OX063-D24) are stable radicals with low toxicity, highly soluble in water, and can be administered systemically through iv injection or directly into the animal tissues. Multiple resonators with horizontal and vertical access are available for in vitro and in vivo small animal oxygen imaging.

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Oxygen Guided Radiation Therapy

Newsletters / November 4, 2020

Spatial heterogeneity in tumor hypoxia is one of the most important factors regulating tumor growth, development, aggressiveness, metastasis, and effective treatment outcome. Most solid tumors are characterized by hypoxia or low oxygen levels (pO2 <10 torr/mmHg) and hypoxic cells have a higher resistance to radiation treatment.

The destruction of hypoxic tumor cells requires approximately 3 times higher radiation dose as compared to well-oxygenated tumor tissues. Hypoxia is also a barrier to successful chemotherapy and immunotherapy treatments as well. Therefore, it is necessary to design tumor treatment that takes accurate tumor pO2 into account. For more information on “Oxygen Guided Radiation Therapy” follow this link.

Oxygen Guided Radiation Therapy Read More »